THE EFFECTIVENESS OF THE APPLICATION OF STABILIZED WATER OZONE FOR THE SANITATION OF BIOAEROSOL AND SURFACES IN CLINICS OF VETERINARY MEDICINE
DOI:
https://doi.org/10.37406/2706-9052-2023-1.30Keywords:
stabilized water ozone, bioaerosol, microflora of veterinary clinics, disinfection efficiencyAbstract
In veterinary clinics for the treatment of small animals, there is a need to use disinfectants for the treatment of various surfaces in order to prevent the spread of pathogenic and opportunistic pathogens. The purpose of the study was to investigate the effectiveness of the antimicrobial effect of stabilized aqueous ozone in the sanitation of bioaerosol and surfaces of boxes for keeping sick animals in veterinary medicine clinics. An ozone generator that produces stable water ozone in a concentration of 1,5 or 3,0 mg/l was used for ozone treatment of bioaerosol and surfaces. The content of mesophilic aerobic microorganisms in the bioaerosol was determined by the sedimentation method, and from the surface of the boxes using swabs. Incubation of crops at a temperature of 30 °C for 72 hours. It was established that the treatment of bioaerosol with stabilized aqueous ozone by the spraying method in the amount of 25–50 ml/m3 of air caused an approximately 40-fold decrease in the number of microorganisms. After this procedure, mesophilic aerobic microorganisms in the amount of no more than 20 CFU/m3 were isolated from the bioaerosol in only 44,4–55,5 % of the samples. Before the bioaerosol was treated with aqueous ozone, the number of mesophilic aerobic bacteria on the surfaces of stainless steel and plastic boxes was in the range of 4,26–4,33 lg CFU/cm2. Wiping surfaces with water and detergent reduced microbial contamination of steel and plastic by 6,2 and 5,6 times, respectively. At the same time, the aerosol application of ozone made it possible to practically destroy microorganisms on the surfaces of the boxes, since no bacteria were released from the washes. The high antimicrobial efficiency of the use of stabilized water ozone for disinfection of tables in veterinary clinics was revealed, both with significant microbial contamination (5 431,5 ± 318,3 CFU/ml of rinse) and with small insemination of surfaces (90–100 CFU/ml of rinse). Since the processing efficiency was 99,9–100 %. Therefore, we suggest using stabilized water ozone for bioaerosol sanitation and table disinfection even during the working day.
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