ANALYSIS OF SENSITIVITY TO ANTIBIOTICS OF SKIN MICROBIOTA OF HEALTHY DOGS AND THOSE WITH PYODERMA
DOI:
https://doi.org/10.37406/2706-9052-2023-4.11Keywords:
pyoderma, antibiotic profile, staphylococci, S. pseudintermedius, causative agents of pyoderma, microbiota.Abstract
Superficial canine pyoderma is a skin infection caused by bacteria such as S. pseudintermedius, S. schleiferi, S. epidermidis, Escherichia coli, and species of the genera Pseudomonas and Proteus. Although it is believed that pyoderma in dogs occurs as a secondary symptom and is a manifestation of the main disease, at the same time, systemic antibiotics are used during the treatment of this purulent-inflammatory process. The purpose of this study was to compare the antibiotic sensitivity of bacteria isolated from the skin of healthy dogs and the main pathogens isolated from pyoderma. Determining the sensitivity of isolated bacteria to antimicrobial drugs was carried out by the method of diffusion in agar using commercial disks in accordance with the CLSI standard. Discs with the following antibiotics were used: amoxicillin with clavulanic acid (20+10 mcg), cefquin (25 mg), ceftriaxone (30 mcg), cephalexin (30 mcg), marbofloxacin (5 mcg), tylosin (50 mcg), tetracycline (30 μg). It was established that the main types of microbiocenosis of the skin of healthy dogs S. pseudintermedius, S. epidermidis and S. saprophyticus were highly sensitive to the beta-lactam antibiotic of the penicillin series amoxicillin with clavulanic acid, cephalosporin antibiotics of the I - IY generation (cephalexin, ceftriaxone and cefquin) and the fluoroquinolone antibiotic marbofloxacin, as the sensitivity of bacteria ranged from 82.3 to 100%. For dog pyoderma, which was registered repeatedly, antibiotic-resistant staphylococci, Escherichia coli and Pseudomonas aeruginosa were detected. The sensitivity of S.pseudintermedius to amoxicillin with clavulanic acid was 58.0%, to cephalosporin antibiotics and marbofloxacin in the range of 64.5–74.2%. A similar trend was observed with determined sensitivity in E.coli and P.aeruginosa, with the exception of the antibiotics tylosin and tetracycline, to which 30–40% of these cultures were sensitive. Therefore, the use of microbiological cultures with the isolation of pathogens and the determination of antimicrobial sensitivity is one of the conditions for reducing the spread of resistant microorganisms and effective treatment. In addition, the search for new highly effective and safe methods of treating infected foci is an alternative for canine pyoderma.
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