IMMUNOBIOLOGICAL REACTIVITY OF COWS WITH CLINICAL INFLAMMATORY PROCESS OF THE MAMMARY GLAND UNDER THE INFLUENCE OF LIPOSOMAL PREPARATION BASED ON ETHYLTHIOSULFANYLATE
DOI:
https://doi.org/10.37406/2706-9052-2024-2.34Keywords:
cow, mastitis, leukocyte profile, liposomal preparation, ethylthiosulfanylate, T- and B-lymphocyte subpopulationsAbstract
Mastitis in cows has a multifactorial nature, which significantly complicates its treatment. One of the factors of mammary gland diseases is bacterial microflora. Given the limited use of antibacterial drugs, it is important to use new alternative antimicrobial agents in the treatment of mastitis. Therefore, the purpose of the study was to determine the effect of a new liposomal drug based on ethylthiosulfanylate on the functional state of immune system factors in cows with clinical mammary inflammation. In the blood of sick cows, an increased number of leukocytes (by 53.3%), pronounced lymphopenia, a decrease in the number of red blood cells and hemoglobin content were found; the content of total protein was 12.1% (p<0.05) lower than in healthy animals. The immune system of sick cows reacts with a decrease in the population of immunocompetent cells. After intramuscular injection of the experimental drug at a dose of 20 ml three times with an interval of 24 hours on the 7th day after the start of treatment, a decrease in the total number of leukocytes by 22.3% and an increase in the content of total protein in the blood serum by 13.4% (p<0.001) were observed. A decrease in the number of neutrophilic granulocytes and an increase in the number of lymphocytes on the 3rd (p<0.05) and 7th day (p<0.001) of the experiment was recorded, indicating the extinction of the inflammatory process. The administration of the studied liposomal preparation to the sick animals contributed to an increase in the number of T-lymphocytes and an increase in their functional activity, especially these changes were expressed on the 7th day after the administration of the drug. For the treatment of animals with catarrhal mastitis, 2–3 injections were sufficient. The therapeutic effect occurred in 48–96 hours and amounted to 89.9%.
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