PRECLINICAL TRIALS OF THE PROPHYLACTIC PRECAUTION POSTBIOTIC

Abstract

The article describes the laboratory and preclinical studies of the microbiological drug developed by us. Its antibacterial properties were determined at different concentrations of the bacteriocin nisin. Various concentrations of such strains of microorganisms were used as test cultures in laboratory studies of antimicrobial activity Escherichia coli, Bacillus cereus, Staphylococcus aureus, Listeria ivanovii, Yersinia enterocolitica. The mechanism of biological action of bacteriocins is primarily related to disruption of the cytoplasmic membranes of microorganisms sensitive to them. Unlike antibiotics, which act rather selectively, bacteriocins also affect antibiotic-resistant strains of microorganisms, are completely broken down and excreted from the body. Bacteriocin nisin is produced by a strain of microorganisms Lactococcus lactis, has antibacterial properties against a wide range of pathogenic microorganisms, is used as a preservative in the food industry. When used internally, lactic acid has an anti-fermenting, antiseptic, and irritating effect. Suppresses the growth and development of conditionally pathogenic and putrefactive microflora of the gastrointestinal tract, stimulates the process of restoration of intestinal villi, which increases the surface area for absorption of nutrients. The test of the second experimental sample on mice showed that the drug did not cause local irritation, skin resorptive and sensitizing effects, did not cause clinical changes and disorders in the work of organ systems of mice. The developed and tested drug is promising for the correction of the endomicroflora of the alimentary canal of animals, including for organic farming, the most promising, in our opinion, are the drugs of microbiological origin.