ASSESSMENT OF BACTERICIDAL ACTION OF AQUEOUS OZONE TO DISINFECTED THE SURFACE OF EGGS
DOI:
https://doi.org/10.37406/2706-9052-2025-2.18Keywords:
water form of ozone, bactericidal action, egg disinfection, bacteria strainsAbstract
Most egg shell contaminants occur immediately after the eggs are laid, and the main source of contamination is the contact of the shell with dirty surfaces. Therefore, the use of ecological safe and effective ways of decontamination of the surface of chicken eggs in the technology of egg production technology, the question is relevant, and the development is innovative and are quite promising. The purpose of this study was to determine the minimal bactericidal concentration and the protein index of aqueous ozone relative to museum strains of microorganisms. It was established that the most resistant to the aqueous form of ozone were spore -forming bacteria B. subtilis, for which minimal bactericidal concentration ozone was 1.53 mg/l, in the second place were yeast C. albicans – minimal bactericidal concentration – 1.23 mg/l, the average minimal bactericidal concentration was registered to S. aureus, E. coli and P. aeruginosa – 0.8 mg/l. The lowest bactericidal concentration (0.8-1.0 mg/l) of aqueous ozone on strains of conditionally pathogenic bacteria was manifested during an exposure of 12–15 min. For the destruction of E. coli and P. aeruginosa cells within one minute, it is necessary that the ozone concentration is 1.23 mg/l, and for S. aureus – 1.53 mg/l. Also, the bactericidal concentration of ozone on strains B. subtilis and C. albicans within 12–15 min of the exposure was 1.53 and 1.23 mg/l, respectively. Organic contamination naturally reduces the bactericidal concentration of ozone, in particular within three minutes of the exposure, on average 1.6 times, and for exposures for six minutes about 1.2 times. That is, to increase the efficiency of the water form of ozone in production conditions it is necessary to increase its concentration and time of influence. At the same time, in order to optimize the concentration of the water form of ozone and contact with microorganisms, it is necessary to carry out more thorough studies to establish all factors that can affect the disinfection process.
References
Albert S., Amarilla A. A., Trollope B., Sng J. D., Setoh Y. X., Deering N., Khromykh A. A.Assessing the potential of unmanned aerial vehicle spraying of aqueous ozone as an outdoor disinfectant for SARS-CoV-2. Environmental Research. 2021. Vol. 196. P. 110944. https://doi.org/10.1016/j.envres.2021.110944
Aslam R., Alam M. S., Saeed P. A. Sanitization Potential of Ozone and Its Role in Postharvest Quality Management of Fruits and Vegetables. Food Engineering Reviews. 2019. Vol. 12, no. 1. P. 48–67. https://doi.org/10.1007/s12393-019-09204-0
Chandran S., Baker C. A., Hamilton A. N., Dhulappanavar G. R., Jones S. L., Gibson K. E. Aqueous Ozone Efficacy for Inactivation of Foodborne Pathogens on Vegetables used in Raw Meat-Based Diets for Companion Animals. Journal of Food Protection. 2023. P. 100175. https://doi.org/10.1016/j.jfp.2023.100175
Czekalski N., Imminger S., Salhi E., Veljkovic M., Kleffel K., Drissner, D., Von Gunten U. Inactivation of Antibiotic Resistant Bacteria and Resistance Genes by Ozone: From Laboratory Experiments to Full-Scale Wastewater Treatment. Environmental Science & Technology. 2016. Vol. 50, no. 21. P. 11862–11871. https://doi.org/10.1021/acs.est.6b02640
El Alami El Hassani N., Baraket A., Alem C. Recent advances in natural food preservatives: a sustainable solution for food safety and shelf life extension. Journal of Food Measurement and Characterization. 2024. Vol. 19. P. 293–315. https://doi.org/10.1007/s11694-024-02969-x
Epelle E. I., Macfarlane A., Cusack M., Burns A., Amaeze N., Richardson K., Yaseen M. Stabilisation of Ozone in Water for Microbial Disinfection. Environments. 2022. Vol. 9, no. 4. P. 45. https://doi.org/10.3390/environments9040045
Feng L., Zhang K., Gao M., Shi C., Ge C., Qu D., Han J. Inactivation of Vibrio parahaemolyticus by Aqueous Ozone. Journal of Microbiology and Biotechnology. 2018. Vol. 28, no. 8. P. 1233–1246. https://doi.org/10.4014/jmb.1801.01056
Girgin Ersoy Z., Barisci S., Dinc O. Mechanisms of the Escherichia coli and Enterococcus faecalis inactivation by ozone. LWT. 2019. Vol. 100. P. 306–313. https://doi.org/10.1016/j.lwt.2018.10.095
Glowacz M., Colgan R., Rees D. The use of ozone to extend the shelf-life and maintain quality of fresh produce. Journal of the Science of Food and Agriculture. 2014. Vol. 95, no. 4. P. 662–671. https://doi.org/10.1002/jsfa.6776
Hamid Z., Meyrick B. K., Macleod J., Heath E. A., Blaxland J. The application ozone within the food industry, mode of action, current and future applications, and regulatory compliance. Letters in Applied Microbiology. 2024. Vol. 77, no. 11. P 101. https://doi.org/10.1093/lambio/ovae101
Kouam M. K., Biekop M. H., Katte B., Teguia A. Salmonella status of table eggs in commercial layer farms in Menoua Division, West region of Cameroon. Food Control. 2018. Vol. 85. P. 345–349. https://doi.org/10.1016/j.foodcont.2017.09.037
Kozlovska I. M., Romanjuk N. Y., Romanjuk L. M., Kukhtyn M. D., Horiuk Y. V., Karpyk G. V. The effect of antimicrobial agents on planktonic and biofilm forms of bacteria that are isolated from chronic anal fissures. Regulatory Mechanisms in Biosystems. 2017. Vol. 8, no. 4. P. 577–582. https://doi.org/10.15421/021789
Kukhtyn M., Horiuk Y., Yaroshenko T., Laiter-Moskaliuk S., Levytska V.. Reshetnyk A. Effect of lactic acid microorganisms on the content of nitrates in tomato in the process of pickling. Eastern-European Journal of Enterprise Technologies. 2018. Vol. 1, no. 11 (91). P. 69–75. https://doi.org/10.15587/1729-4061.2018.120548
Kukhtyn M., Sverhun Z., Horiuk Y., Salata V., Laiter-Moskaliuk S., Mocherniuk M., Horiuk V. The influence of different methods of decontamination of microbial biofilms formed on eggshells. Potravinarstvo Slovak Journal of Food Sciences. 2024. Vol. 18. P. 666–682. https://doi.org/10.5219/1981
Lin C. M., Chen S. Y., Lin Y. T., Hsiao C. P., Liu C. T., Hazeena S. H., Hou C. Y. Inactivating Salmonella Enteritidis on shell eggs by using ozone microbubble water. International Journal of Food Microbiology. 2023. P. 110213. https://doi.org/10.1016/j.ijfoodmicro.2023.110213
Miller F. A., Silva C. L. M., Brandão T. R. S. A Review on Ozone-Based Treatments for Fruit and Vegetables Preservation. Food Engineering Reviews. 2013. Vol. 5, no. 2. P. 77–106. https://doi.org/10.1007/s12393-013-9064-5
Mochernyuk M. M., Kukhtyn M. D., Horiuk Y. V., Danilkov S. O. Effectiveness of using stabilized aqueous ozone for bioaerosol and surface sanitation in veterinary clinics. Podilian Bulletin Agriculture Engineering Economics. 2023. Vol. 38. С. 203–209. https://doi.org/10.37406/2706-9052-2023-1.30
Mohammadi-Aragh M. K., Linhoss J. E., Evans J. D. Effects of various disinfectants on the bacterial load and microbiome of broiler hatching eggs using electrostatic spray. Journal of Applied Poultry Research. 2022. P. 100278. https://doi.org/10.1016/j.japr.2022.100278
Ölmez H., Kretzschmar U. Potential alternative disinfection methods for organic fresh-cut industry for minimizing water consumption and environmental impact. LWT – Food Science and Technology. 2009. Vol. 42, no. 3. P. 686–693. https://doi.org/10.1016/j.lwt.2008.08.001
Patil S., Cullen P. J., Kelly B., Frias J. M., Bourke P. Extrinsic control parameters for ozone inactivation ofEscherichia coliusing a bubble column. Journal of Applied Microbiology. 2009. Vol. 107, no. 3. P. 830–837. https://doi.org/10.1111/j.1365-2672.2009.04255.x
Pavlovich M. J., Chang H. W., Sakiyama Y., Clark D. S., Graves D. B. Ozone correlates with antibacterial effects from indirect air dielectric barrier discharge treatment of water. Journal of Physics D: Applied Physics. 2013. Vol. 46, no. 14. P. 145202. https://doi.org/10.1088/0022-3727/46/14/145202
Perry J. J., Yousef A. E. Decontamination of Raw Foods Using Ozone-Based Sanitization Techniques. Annual Review of Food Science and Technology. 2011. Vol. 2, no. 1. P. 281–298. https://doi.org/10.1146/annurev-food-022510-133637
Sarron E., Gadonna-Widehem P., Aussenac T. Ozone Treatments for Preserving Fresh Vegetables Quality, A Critical Review. Foods. 2021. Vol. 10, no. 3. P. 605. https://doi.org/10.3390/foods10030605
Savitri D., Shetty S., Chandra S. S., Jayalakshmi K. B., Gowda M., Rai N., Reddy S. Efficacy of ozonated water, 2% chlorhexidine and 5.25% sodium hypochlorite on five microorganisms of endodontic infection: In vitro study. Advances in Human Biology. 2018. Vol. 8, no. 1. P. 19. https://doi.org/10.4103/aihb.aihb_45_16
Song W. J., Shin J. Y., Ryu S., Kang D. H. Inactivation of Escherichia coli O157:H7, Salmonella Typhimurium and Listeria monocytogenes in apple juice at different pH levels by gaseous ozone treatment. Journal of Applied Microbiology. 2015. Vol. 119, no. 2. P. 465–474. https://doi.org/10.1111/jam.12861
Tizaoui C. Ozone: A Potential Oxidant for COVID-19 Virus (SARS-CoV-2). Ozone: Science & Engineering. 2020. Vol. 42, no. 5. P. 378–385. https://doi.org/10.1080/01919512.2020.1795614
Wan Z., Chen Y., Pankaj S. K., Keener K. M. High voltage atmospheric cold plasma treatment of refrigerated chicken eggs for control of Salmonella Enteritidis contamination on egg shell. LWT – Food Science and Technology. 2017. Vol. 76. P. 124–130.
https://doi.org/10.1016/j.lwt.2016.10.051
Zeweil H. S., Rizk R. E., Bekhet G. M., Ahmed M. R. Comparing the effectiveness of egg disinfectants against bacteria and mitotic indices of developing chick embryos. The Journal of Basic & Applied Zoology. 2015. Vol. 70. P. 1–15. https://doi.org/10.1016/j.jobaz.2014.12.005